The free fatty acids bound to human serum albumin

In vitro use of free fatty acids bound to albumin: A comparison of protocols.

The metabolism of albumin-bound C14-labeled unesterified fatty acids in normal human subjects.

Recent studies have indicated that the unesterified fatty acids in plasma (UFA) may represent a major form in which fat is transported to tissues for utilization as a source of energy. The evidence indicating the metabolic importance of UFA includes a close correlation between plasma UFA concentration and nutritional state (1, 2), direct evidence of removal of UFA by the heart and liver in fast...

Binding of indole analogues to human serum albumin. Effects of fatty acids.

In earlier studies on the binding of indole analogues with defatted human serum albumin (l), the intrinsic association constants for both a neutral ligand and a ligand bearing a negative charge were found to be invariant in the pH region of 5 to 10, implying that the structure of the binding site on the albumin did not change in this region. As the pH increased or decreased from this region the...

Removal of fatty acids from serum albumin by charcoal treatment.

The fatty acid contents of 26 different serum albumin preparations representing different species and obtained from various commercial sources have been determined. Some samples had surprisingly little fatty acid contamination, but it was found that other samples contained between 2 and 3 moles of acid per mole of protein, in confirmation of earlier reports. Treatment of these samples with char...

Albumin-associated free fatty acids induce macropinocytosis in podocytes.

Podocytes are specialized epithelial cells in the kidney glomerulus that play important structural and functional roles in maintaining the filtration barrier. Nephrotic syndrome results from a breakdown of the kidney filtration barrier and is associated with proteinuria, hyperlipidemia, and edema. Additionally, podocytes undergo changes in morphology and internalize plasma proteins in response ...